更新时间:2024-08-08
Antigen:AntibodiesforMousePlateletDepletionDescription:ThisantibodypreparationcontainsamixtureofpurifiedratmonoclonalantibodiesdirectedagainstmouseGPIbalpha(CD42b)
Antigen: Antibodies for Mouse Platelet Depletion
Description: This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb alpha (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice.
Clone: polyclonal anti-GPIb alpha
Isotype: Rat IgG
Form: purif.
Applications: platelet depletion
Size: 0.5 mg
德国Emfret公司专注心血管和血液系统研究用抗体研发和生产,供应的抗体包括清除血液中血小板、流式细胞术鉴定分析小鼠血小板表面糖蛋白,Emfret公司的抗体不仅适用于流失分析、WB检测,还可用于免疫组化/免疫荧光及免疫共沉淀检测。靶向小鼠血小板清除的抗体,是混合物,包含抗小鼠 GPIbα (CD42b) 的纯化大鼠单克隆抗体。 用二价 IgG 靶向该受体会导致小鼠中严重且不可逆的 Fc 非依赖性血小板清除。 血小板清除经常应用于研究血小板的(病理)生理学中的全面作用。
Depletion of mouse platelets by anti-GPIba(R300) treatment
Wild type mice received 2 or 4 µg/g platelet depletion antibodies in 200 µl sterile PBS intravenously and platelet counts were determined at the indicated time points. Results are mean±s.d. of 6 mice per group.
Specificity: This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb(CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice1,2. Platelet depletion is frequently used to study the general role of platelets in (patho-) physiology3.
Preparation and Storage: The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography and contain < 0.00025 ng/μg (< 0.0025 EU/μg) protein endotoxin. Stable for six months from date of shipment when stored at 4°C. KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.
Usage: This preparation is optimized to reduce the platelet count by >95% within 60 minutes after intravenous injection of 2 μg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 μl (diluted with sterile PBS).