中文介绍:
红色荧光 TAMRA 叠氮化物 (TAMRA-PEG4-Azide) 可以通过铜催化的点击反应 (CuAAC) 与末端炔烃反应。 它还通过无铜“点击化学"反应与应变的环辛炔反应形成稳定的三唑,并且不需要铜催化剂或高温。
TAMRA(四甲基罗丹明)是一种明亮的荧光标记,与各种激发源兼容,包括汞弧、钨和氙弧灯、氦氖激光器的 544 nm 线和 532 nm 绿色激光线。
英文介绍:
The red-fluorescent TAMRA Azide (TAMRA-PEG4-Azide) can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry" reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
TAMRA (tetramethylrhodamine) is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.
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参考文献:
1. David, L. L., et al. (2021). Chemical Proteomics Approach for Profiling the NAD Interactome. J Am Chem Soc., 143 (18), 6787-6791.