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27-炔基胆固醇27-Alkyne Cholesterol货号1401-Click Chemistry Tools

更新时间:2024-02-25   点击次数:329次

ClickChemistryTools产品27-Alkyne cholesterol英文介绍

27-Alkyne cholesterol is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper-containing catalyst.

Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

27-Alkyne cholesterol is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria.

In summary, alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.


ClickChemistryTools产品27-Alkyne cholesterol中文介绍

27-炔烃胆固醇是一种含有欧米茄末端炔烃的改性脂质。在含铜催化剂存在下,末端炔烃基可用于与含叠氮化物试剂的高度特异性

连接反应,称为“点击化学"。

炔烃胆固醇是一种多功能、灵敏且易于使用的工具,用于跟踪细胞胆固醇代谢和定位,因为它允许多种检测方法,包括质谱和荧光

显微镜。

27-炔烃胆固醇被来自不同生物物种(短杆菌、酵母、大鼠、人)的细胞酶所接受,这些酶包括胆固醇氧化酶、羟化酶和酰基转移

酶,它们在体外和体内测定中产生预期的代谢物。使用荧光显微镜,研究人员可以在亚细胞分辨率下研究胆固醇的分布,检测高尔

基体和质膜中的脂质,还可以检测内质网和线粒体中的脂质。

总之,炔烃胆固醇是一种多功能、灵敏且易于使用的工具,用于跟踪细胞胆固醇代谢和定位,因为它允许多种检测方法,包括质谱

和荧光显微镜。

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Selected References

1.Friedrich, F., et al. (2021). Direct Measurement of Intermolecular Mechanical Force for Nonspecific Interactions between Small Molecules. J Phys Chem Lett., 12 (46), 11316-11322.

2.Hofmann K., et al. (2014). A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. J. Lipid. Res., 55, 583-91.

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