中文摘要：
为了研究卵巢巨噬细胞群体在排卵中的作用，我们研究了使用脂质体封装的氯膦酸盐消耗该群体的影响。在排卵前 84 小时（第 -3 天）或 36 小时（第 -1 天）在促性腺激素引发的成年小鼠的卵巢滑囊下注射氯膦酸脂质体、盐水脂质体或单独生理盐水。排卵率是通过计算释放的卵母细胞数量来确定的。排卵前后也立即计算了graafian卵泡和黄体的数量。使用针对巨噬细胞抗原 macrosialin、主要组织相容性复合物 II 类 （Ia） 和 F4/80 的特异性单克隆抗体进行免疫组化检查排卵前卵巢的 theca 和基质内的巨噬细胞分布。在第 -1 天注射氯膦酸脂质体不影响排卵率，而在第 -3 天注射氯膦酸脂质体导致排卵率显着降低（平均排卵卵母细胞分别为 5. 25 +/- 0.6 来自氯膦酸脂质体处理的卵巢和 9.13 +/- 0.9 来自盐水处理的卵巢，P < 0.05）。排卵时 theca 中存在的巨唾液酸阳性巨噬细胞的数量通过第 -1 天的氯膦酸脂质体处理减少，第 -3 天的治疗减少了 theca 中存在的 Ia 阳性和巨唾液素阳性巨噬细胞的数量。当通过阴道涂片检查随后的卵巢周期时，发现氯膦酸脂质体处理的动物的中胚层-2/二元期延长（7.5 +/- 1.3天，盐水脂质体处理动物为3.4 +/- 0.4天，P < 0.05）。这些结果表明，鞘状巨噬细胞可能参与卵泡生长和破裂的调节，并且对发情周期的正常进展很重要。
英文摘要：
To investigate the role of the ovarian macrophage population in ovulation, we examined the effect of depleting this population using liposome-encapsulated clodronate. Clodronate liposomes, saline liposomes, or saline alone was injected under the ovarian bursa in gonadotropin-primed adult mice, either 84 h (Day -3) or 36 h (Day -1) before ovulation. Ovulation rates were determined by counting the number of oocytes released. The numbers of graafian follicles and corpora lutea were also counted immediately before and after ovulation. Macrophage distribution within the theca and stroma of preovulatory ovaries was examined by immunohistochemistry with specific monoclonal antibodies to the macrophage antigens macrosialin, major histocompatability complex class II (Ia), and F4/80. Injection of clodronate liposomes on Day -1 did not affect ovulation rates, whereas administration on Day -3 caused a significant reduction in ovulation rate (mean oocytes ovulated = 5. 25 +/- 0.6 from clodronate liposome-treated ovaries and 9.13 +/- 0.9 from saline-treated ovaries, respectively, P < 0.05). The numbers of macrosialin-positive macrophages present in the theca at ovulation were reduced by treatment with clodronate liposomes on Day -1, and treatment on Day -3 reduced the numbers of Ia-positive and macrosialin-positive macrophages present in the theca. When the subsequent ovarian cycles were examined by vaginal smearing, the metestrous-2/diestrous stage was found to be extended in clodronate liposome-treated animals (7.5 +/- 1.3 days vs. 3.4 +/- 0.4 days for saline liposome-treated animals, P < 0.05). These results suggest that thecal macrophages may be involved in the regulation of follicular growth and rupture, as well as being important for the normal progression of the estrous cycle.
产品详情：
品牌：LIPOSOMA
货号：CP-005-005
产地：荷兰
品名：Clodronateliposomes氯膦酸二钠脂质体巨噬细胞清除剂
实验细节：
实验结果：
论文题目：
该文章比较久了，是较早研究卵巢巨噬细胞的文章，可以借鉴。因为研究很早，所以数据很基础。无论如何，我们可以订购荷兰liposoma品牌的巨噬细胞清除剂Clodronateliposomes，具体订购联系靶点科技。这为研究清除卵巢巨噬细胞提供的研究基础。
