中文摘要：
天生的防御反应虽然主要是本能的，但也必须高度适应风险评估的变化。然而，在严重压力之后，适应性变化可能会变得适应不良，如创伤后应激障碍 （PTSD） 所见。在一系列实验中，我们观察到雄性小鼠在不良休克经历后对先前无威胁的刺激的先天逃避行为的长期变化表现为威胁反应阈值的转变。通过记录上丘 （SC） 中的神经活动，同时对传入神经的特定反应进行光标记，我们确定了从内侧前额叶皮层 （mPFC） 到达 SC 的输入对不良休克经历后逃逸相关活动的直接和间接影响。在冲击期间停用这些特定投影有效地消除了观察到的变化。相反，在遭遇期间光遗传学激活它们会控制逃逸反应。这确定了那些特定的 mPFC 输入到 SC 中对于先天逃脱行为的不良体验相关变化的必要性和充分性。
英文摘要：
Innate defensive responses, though primarily instinctive, must also be highly adaptive to changes in risk assessment. However, adaptive changes can become maladaptive, following severe stress, as seen in posttraumatic stress disorder (PTSD). In a series of experiments, we observed long-term changes in innate escape behavior of male mice towards a previously non-threatening stimulus following an adverse shock experience manifested as a shift in the threshold of threat response. By recording neural activity in the superior colliculus (SC) while phototagging specific responses to afferents, we established the crucial influence of input arriving at the SC from the medial prefrontal cortex (mPFC), both directly and indirectly, on escape-related activity after adverse shock experience. Inactivating these specific projections during the shock effectively abolished the observed changes. Conversely, optogenetically activating them during encounters controlled escape responses. This establishes the necessity and sufficiency of those specific mPFC inputs into the SC for adverse experience related changes in innate escape behavior.
论文信息：
论文题目：Prefrontal control of superior colliculus modulates innate escape behavior following adversity
期刊名称：Nature Communications
时间期卷：15, Article number: 2158 (2024)
在线时间：2024年3月9日
DOI：doi.org/10.1038/s41467-024-46460-z
产品信息：
货号1：R180
品名1：Red fluorescent tracer microspheres：Red Retrobeads™ IX
货号2：G180
品名1：Green fluorescent tracer microspheres：Green Retrobeads™ IX
规格：100ul
品牌：Lumafluor
产地：美国
名称：fluorescent tracer microspheres
现货授权：Target Technology（靶点科技）
Lumafluor助力研究上丘的前额叶控制调节逆境后的先天逃逸行为文章截图
Lumafluor助力研究上丘的前额叶控制调节逆境后的先天逃逸行为，产品使用截图。
For anatomical tracing of SC afferents and DMS afferents, Green and Red fluorescent tracer microspheres (Retrobeads, Lumafluor) were injected into the SC (AP:−3.80 mm, ML: ± 0.8 mm, DV:−1.5 mm) and into the DMS (AP: + 0.5 mm, ML: ± 1.25 mm, DV-3mm). The stock solution was diluted in pbs (1/4) and the volume per injection area was 600 μL.
