小鼠肿瘤接种模型，时间一般持续2-3周。如何使用氯膦酸二钠脂质体清除小鼠肿瘤模型巨噬细胞？对于这种时间长的疾病模型，小鼠的状态，小鼠的存活，比清除效果更为重要。两者的平衡或者偏向，需要动态调整。
如下这篇Nature Communications，使用荷兰Liposoma品牌巨噬细胞清除剂氯膦酸二钠脂质体：Clodronate Liposomes&Control Liposomes。小鼠疾病模型：LLC肺癌模型。LLC细胞全称为Lewis肺癌细胞（Lewis Lung Carcinoma），是一种来源于小鼠的肺癌细胞系，常用于癌症研究的动物模型中。它并非人类肺癌的临床分型，而是实验室中用于模拟肺癌生长、转移及药物测试的模型工具。腹腔注射200ul，在肿瘤细胞接种后第7天，11天，15天腹腔注射荷兰Liposoma品牌巨噬细胞清除剂氯膦酸二钠脂质体：Clodronate Liposomes&Control Liposomes（CP-005-005）。
氯膦酸二钠脂质体清除小鼠肿瘤模型巨噬细胞效果检测：
a Schematic of clodronate and control liposome treatment of LLC tumor bearing mice. b, c LLC tumor volumes over time (b) and endpoint tumor volumes (c) for WT (n = 6 mice treated with control liposomes (con) and 8 mice treated with clodronate liposomes (clod) and for Mlck210−/− [n = 7 mice treated with control liposomes (con) and clodronate liposomes (clod)]. One WT animal treated with clodronate liposomes died on day 14 post-tumor inoculation and was not included in the final tumor weight graph. d–g Quantification (d, f) and FACs plots of Ly6C- (e) liver and (g) tumor macrophages from WT and Mlck210−/− mice after treatment with clodronate and control liposomes (n = 4 mice).
论文信息：
论文题目：PI3Kγ stimulates a high molecular weight form of myosin light chain kinase to promote myeloid cell adhesion and tumor inflammation
期刊名称：Nature Communications
时间期卷：13, Article number: 1768 (2022)
在线时间：2022年4月1日
DOI：doi.org/10.1038/s41467-022-29471-6
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：Target Technology(靶点科技)
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法：
In other experiments, wildtype female C57BL6 or Mlck210−/− mice in the C57BL6 background bearing LLC tumors (n = 6–8) were treated with daily i.p. injections of 1 mg clodronate or control liposomes (Liposoma Research Liposomes # CP-005-005) in 200 µl on day 7,11 and 15 after tumor inoculation. Tumors dimensions were recorded at regular intervals, typically every 1–2 days. Tumors were excised at 18 days after implantation and tumors, spleens and livers were excised for further analysis by flow cytometry. Alternatively, WT and Mlck210−/− C57Bl6 male mice bearing HPV+ MEER tumors (n = 8–11) were treated with i.p. injections of 100 µg anti-CD8 antibodies (BioXcell In Vivo Plus Clone YTS 169.4, #BE0117) or saline on days 27, 29, 32, 41, and 44 after tumor inoculation. Tumor volumes were measured every 2–3 days. Tumors and spleens were harvested on day 48 after tumor inoculation for flow cytometry analysis.
