中文摘要：
炎症通常会导致单核细胞来源的巨噬细胞募集。这些细胞的命运以及它们在多大程度上能够获得常驻巨噬细胞的身份和功能仍不清楚。在这里，我们显示，在轻度炎症期间引入腹腔的巨噬细胞可以长期存活，但由于持续存在的常驻巨噬细胞的存在，它们会保持在未成熟的过渡分化状态。相比之下，严重炎症会导致常驻巨噬细胞的消失，并出现一个延长阶段，此阶段下腹腔无法维持常驻表型，但最终引入的细胞能够获得成熟的常驻身份。这些巨噬细胞在转录和功能上也表现出差异，这些差异源于炎症对腹腔微环境的改变，并在较小程度上受其来源和驻留时间的影响。因此，炎症引发的腹腔巨噬细胞的命运似乎不是预先决定的，而是由环境所调节。
英文摘要：
Inflammation generally leads to recruitment of monocyte-derived macrophages. What regulates the fate of these cells and to what extent they can assume the identity and function of resident macrophages is unclear. Here, we show that macrophages elicited into the peritoneal cavity during mild inflammation persist long-term but are retained in an immature transitory state of differentiation due to the presence of enduring resident macrophages. By contrast, severe inflammation results in ablation of resident macrophages and a protracted phase wherein the cavity is incapable of sustaining a resident phenotype, yet ultimately elicited cells acquire a mature resident identity. These macrophages also have transcriptionally and functionally divergent features that result from inflammation-driven alterations to the peritoneal cavity micro-environment and, to a lesser extent, effects of origin and time-of-residency. Hence, rather than being predetermined, the fate of inflammation-elicited peritoneal macrophages seems to be regulated by the environment.
论文信息：
论文题目：Recruited macrophages that colonize the post-inflammatory peritoneal niche convert into functionally divergent resident cells
期刊名称：Nature Communications
时间期卷：12, Article number: 1770 (2021)
在线时间：2021年3月19日
DOI：doi.org/10.1038/s41467-021-21778-0
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes
办事处：Target Technology(靶点科技)
Clodronate Liposomes氯膦酸盐脂质体清除肝脏和肿瘤巨噬细胞，疾病模型为：肺癌模型Lewis lung carcinoma (LLC)。荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes见刊于Nature Communications：被招募的巨噬细胞在炎症后腹膜微环境中定殖，会转化为功能上不同的常驻细胞。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法：
Sterile peritoneal inflammation
To elicit sterile peritoneal inflammation, mice were injected with 10 or 1000 µg of zymosan A (Sigma-Aldrich) suspended in 200 µl Dulbecco’s PBS (Invitrogen), dPBS or left naïve as indicated. In some experiments, mice were injected intraperitoneally with 250 µl of 700 nm PKH26-PCL in suspended in Diluent B (Sigma) 24 h prior to zymosan. In some experiments, mice were injected intraperitoneally with 0.0625 mg Clodronate liposomes (Liposoma) suspended in 250 µl dPBS (Gibco). To elicit LPS-induced inflammation mice were injected intraperitoneally with 5 µg of LPS (O111:B4, Sigma-Aldrich) suspended in 200 µl dPBS.
材料和方法文献截图：
