圈门策略：
小鼠心脏细胞流式分选或分析需根据细胞类型、实验目的选择合适的圈门策略。以下是关键要点和方案。
巨噬细胞圈门策略：
表面标志物选择：CD11b（单核/巨噬细胞标志）结合F4/80（巨噬细胞特异性抗原）可有效区分巨噬细胞群体。
散射参数：前向散射（FSC）和侧向散射（SSC）可辅助筛选细胞大小和复杂度，排除杂质细胞。
补偿调节：需对FITC、PE等荧光染料进行补偿设置，避免信号溢漏导致假阳性。
论文信息：
论文题目：Cardiac macrophages prevent sudden death during heart stress
期刊名称：Nature Communications
时间期卷：12, Article number: 1910 (2021)
在线时间：2021年3月26日
DOI：doi.org/10.1038/s41467-021-22178-0
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes& Control liposomes
办事处：Target Technology(靶点科技)
Clodronate Liposomes氯膦酸盐脂质体清除肝脏和肿瘤巨噬细胞，疾病模型为：肺动脉环缩术(pulmonary artery banding, PAB)模型。Pulmonary artery banding (PAB) 是通过环束带限制肺动脉血流的姑息性手术，主要用于治疗先天性心脏病，如室间隔缺损、大动脉转位等伴随过度肺血流的疾病。 原理: 该手术通过聚四氟乙烯带环束肺动脉主干，降低肺动脉压力和血流量，缓解心脏负担。早期作为复杂先心病分期治疗的过渡手段，现仅用于多发性室间隔缺损、合并主动脉缩窄等特殊病例。荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes见刊于Nature Communications：心脏巨噬细胞在心脏压力期间防止猝死。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法文字描述：
Male C57BL6/J mice were purchased from CLEA Japan and Jackson Laboratory and maintained on a standard mouse chow diet under sterile barrier conditions, on a 12-h light-dark cycle with 18–23 °C and 40–60% humidity. Male Areg homozygous null (Areg−/−) mice were backcrossed to C57BL6/J mice over ten generations. Rag2−/−, Cd4−/−, and Cd8a−/− mice were purchased from Taconic (Germantown, NY). The genotypes of all mice were determined using genomic PCR. The isoproterenol challenge entailed bolus administration of isoproterenol (Sigma) 5 mg/kg intraperitoneally under constant ECG recording. Clodronate liposomes and control liposomes were purchased from LIPOSOMA B.V. A total of 10 μl of clodronate or control liposome solution per gram of mouse were intravenously administrated 24 h before PAB. After the first administration of clodronate or control liposomes, the same volume of liposomes was also administrated every 7 days for long term depletion, per the manufacturer’s instructions. All protocols for animal experiments were approved by the Animal Care and Use Committee of the University of Tokyo.
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法文献截图：
