Doccol 线拴是国际脑缺血研究中常用线拴，是 Nature，Stroke，J Neurosci 等杂志报道最多的线拴。根据谷歌学术的搜索结果，截止至目前，已经有数千篇研究脑缺血的文献引用了 Doccol 线栓。仅 2024 年 1 月至 12 月就有 347 篇，文献目录见我们的网站。美国的 Doccol 线拴是脑缺血 MCAO 模型稳定的保证，可将脑梗塞体积标准差减少至 5%，并避免蛛网膜下腔出血，不需要激光多普勒检测血流也能获得极为稳定的结果。同时，每只鼠 MCAO 的线栓成本可能仅为 30-50 元（可重复使用型号，视重复使用次数决定）。靶点科技是 Doccol 线栓中国总代理，大鼠和小鼠常用型号的栓线都是现货，Doccol线栓MCAO栓线脑缺血线栓选购指南：
Doccol MCAO 线拴可以将脑梗塞体积标准差减少至 5%成功的 MCAO 模型的脑血流在插入线拴时会有断崖式下降；在拔出线拴后，激光多普勒检测的血流会很快恢复。采用硬质尼龙鱼线线拴，极易戳破血管，造成蛛网膜下腔出血（Subarachnoid hemorrhage·）。手术过程中血管如果被线拴戳破，脑血流不会有断崖式下降，而是缓慢下降；在拔出线拴后，脑血流也不会恢复。造成脑梗塞体积变异的原因还有，不充分的梗塞血管或线拴移位造成的血流下降程度不均一及提前灌注。因此，线栓的质地是 MCAO 模型稳定的关键。
对于 MCAO 模型来说，主要有两种线拴，无包被的火焰熔烫圆头线拴及硅胶包被线拴。研究表明 (Tsuchiya et al. 2003)，无包被的圆头线拴在 MCAO 实验过程中可能导致高达 40% 的蛛网膜下腔出血，而其标准差可能比平均值的 50% 还要大。另一项技术文章表明 (Shimamura et al. 2006a)，硅胶包被线拴要远好于无包被的圆头线拴，其结果更为稳定，即使对于没有经验的实验者来说也是如此。而多聚赖氨酸包被的线拴只能增加死亡率，不能减少梗赛体积的变异程度 (Huang et al. 1998)。
Doccol MCAO 线拴全部为硅胶包被，圆润、柔软、包被均匀，即使在没有激光多普勒实时检测的情况，也不会将血管戳破。使用自制的硅胶包被的线拴，大鼠 (Schmid-Elsaesser et al. 1998) 及小鼠 (Shah et al. 2006) 的脑梗塞体积的标准差均在 30% 左右。而使用 Doccol MCAO 线拴可以得到更好的结果。对于大鼠 MCAO 模型来说，使用 Doccol MCAO 线拴得到的结果的标准差可达 10%-20% （Ruscher et al.2012；Ishizaka et al.2013；Sakata et al.2012；Candelario-Jalil et al. 2008；Khan et al. 2006；Liu et al. 2006；Shimamura et al. 2006b；Solaroglu et al. 2006；Tsubokawa et al. 2007；Tsubokawa et al. 2006a；Tsubokawa et al. 2006b)。对于小鼠来说，使用 Doccol MCAO 线拴得到的结果的标准差可达 5%-10%(Chen et al.2014; Bae et al.2013; Jin et al.2011; Gu et al.2012; Kleinschnitz et al. 2007; Maysami et al. 2008; Pignataro et al. 2007b; Pignataro et al. 2007c)。
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