肺脏，肝脏，脾脏三个部位经常是动物实验需要检测的部位，如何通过流式检测肺脏，肝脏，脾脏三个部位的巨噬细胞？首要的是流式圈门，肺肝脾巨噬细胞圈门策略文献参考，可以参考如下文献。
论文信息：
论文题目：Microbiota-induced EI24 improves homeostasis but impedes function of alveolar macrophages via metabolic regulation
期刊名称：Nature Communications
时间期卷：17, Article number: 2227(2026)
在线时间：2026年1月31日
DOI： doi.org/10.1038/s41467-026-68466-5
Clodronate Liposomes氯膦酸盐脂质体在小鼠病毒感染和肿瘤转移模型清除肺泡巨噬细胞和外周单核巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于Nature Communications：微生物群诱导的EI24通过代谢调控改善稳态，但阻碍肺泡巨噬细胞功能。
肺肝脾巨噬细胞圈门策略文献参考：
a/肺脏：Gating strategy used for identification of lung AMs and IMs.
AMs were identified as CD45+ CD11b1o CD11c+ F4/80+ SiglecF+
IMs were characterized as CD45+CD11b+CD11c- F4/80+.
b/肝脏： Gating strategy of liver macrophages.
Liver macrophages wereidentified as CD45+ F4/80+ CD11b+
c/脾脏： Gating strategy of spleen RPMs.
RPMs wereidentified as CD45+ F4/80+ CD11bint
