中文摘要：
在全身给药后，影响纳米粒在体内命运的因素仍是一个备受关注的研究领域。其中尤为引人关注的问题是，用癌症特异性抗体配体进行标记（即“主动靶向"）是否优于其未标记的对应物（即“被动靶向"）。利用三种免疫变异小鼠模型的乳腺癌，我们证明，抗体标记纳米粒的瘤内滞留是由肿瘤相关树突状细胞、中性粒细胞、单核细胞和巨噬细胞决定的，而非由抗体-抗原相互作用决定。全身暴露于任何一种纳米粒都会诱导免疫反应，导致CD8+ T细胞浸润和肿瘤生长延迟，且这种效应与抗体的治疗活性无关。这些结果表明，无需治疗性载荷，全身暴露于纳米粒即可诱导抗肿瘤免疫反应。我们的结论是，宿主的免疫状态和实体瘤的微环境是癌症纳米医学研究中的关键变量，并且纳米技术可能具有应用于癌症免疫治疗的潜力。
英文摘要：
The factors that influence nanoparticle fate in vivo following systemic delivery remain an area of intense interest. Of particular interest is whether labeling with a cancer-specific antibody ligand (“active targeting") is superior to its unlabeled counterpart (“passive targeting"). Using models of breast cancer in three immune variants of mice, we demonstrate that intratumor retention of antibody-labeled nanoparticles was determined by tumor-associated dendritic cells, neutrophils, monocytes, and macrophages and not by antibody-antigen interactions. Systemic exposure to either nanoparticle type induced an immune response leading to CD8+ T cell infiltration and tumor growth delay that was independent of antibody therapeutic activity. These results suggest that antitumor immune responses can be induced by systemic exposure to nanoparticles without requiring a therapeutic payload. We conclude that immune status of the host and microenvironment of solid tumors are critical variables for studies in cancer nanomedicine and that nanoparticle technology may harbor potential for cancer immunotherapy.
论文信息：
论文题目：Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
期刊名称：Science Advances
时间期卷：Vol 6, Issue13(2020)
在线时间：2020年3月25日
DOI: 10.1126/sciadv.aay1601
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：靶点科技
Clodronate Liposomes氯膦酸盐脂质体清除小鼠乳腺癌模型里巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于Science Advances：在乳腺癌模型中，纳米颗粒与免疫细胞的相互作用主导了肿瘤滞留，并诱导了T细胞介导的肿瘤抑制。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除巨噬细胞的材料和方法：
In vivo macrophage depletion
Athymic nude mice growing HCC1954 tumors (n = 3 with two tumors each) were treated with two consecutive doses of clodronate liposome (CL) (Liposoma, Netherlands) via intraperitoneal (300 μl per animal) injection. After the second dose of CL, BH nanoparticles were injected (5 mg of Fe per mouse intravenously) and euthanized 24 hours later to harvest tumors for ICP-MS.
巨噬细胞清除材料和方法文献截图：在乳腺癌模型中，纳米颗粒与免疫细胞的相互作用主导了肿瘤滞留，并诱导了T细胞介导的肿瘤抑制。
