Brainbits货号NB4产品说明书-中文
NbActiv4® 神经元培养基是Neurobasal/B27™的改进版本。与Neurobasal/B27相比，NbActiv4 培养的神经元突触数量为后者的两倍，尖峰放电频率最高可达后者的 8 倍。通过在我们的BrainBits（Transnetyx Tissue）基础培养基/NS21中添加3种新成分——肌酸、雌激素和胆固醇——NbActiv4针对最大尖峰放电频率进行了优化。呼吸测量表明，NbActiv4中的神经元处于较小的代谢应激下，并且在谷氨酸需求负荷下运行更高效。NbActiv4是一种方便且实用的无血清神经元培养基，适用于电生理学、发育期突触可塑性、神经元网络分析、药理学、毒理学和药物发现。与Neurobasal/B27相比，NbActiv4中的突触和尖峰活动发育更早，从第1周就开始出现（Brewer et al. 2008. Neuron network activity scales exponentially with synapse density. J. Neural Eng., in press）Brewer,GJ., Boehler, MD; Jones, TT, Wheeler, BC. 2008. NbActiv4 medium for neurons increases synaptic densities and network spike rates on multielectrode arrays compared to Neurobasal/B27. J. Neurosci. meth. 170:181-187 (PM 18308400)
从Neurobasal/B27转换至NbActiv4® 的策略
NbActiv4含有BrainBits（Transnetyx Tissue）基础培养基和 NS21。未开封时可在4 °C下储存最长6个月。不应冷冻。两种培养基（Neurobasal/B27 和 NbActiv4）均设计用于 37 °C、5% CO₂ 培养箱中使用。如果氧气浓度也调节至 9%，神经元受到的氧化应激更小。为了在当前实验背景下评估NbActiv4，我们建议进行平行对照比较。对于新的研究方向，可以直接从NbActiv4开始实验。先在Neurobasal/B27中开始培养、随后转换为NbActiv4，并未显示出比从一开始就使用NbActiv4更好的迹象。应至少每周进行一次半量换液，使用已在培养箱温度下平衡的培养基；当细胞密度高于300个/mm² 时，应更频繁换液。
Brainbits货号NB4产品说明书-英文
NbActiv4® neuron culture medium is an improved version of Neurobasal / B27tm. NbActiv4 grows neurons with twice as many synapses and up to 8x higher spike rates than Neurobasal/B27. NbActiv4 was optimized for maximum spike rates by addition of 3 new components to our BrainBits (Transnetyx Tissue) Basal Medium/NS21: creatine, estrogen and cholesterol. Measures of respiration indicated that neurons in NbActiv4 were under less metabolic stress and operated more efficiently under demand loading from glutamate. NbActiv4 is a convenient and useful serum-free neuron culture medium for electrophysiology, developmental synaptic plasticity, neuronal network analyses, pharmacology, toxicology and drug discovery. Synapses and spiking activity develop earlier, starting at 1 week in NbActiv4 compared to Neurobasal/B27 (Brewer et al. 2008. Neuron network activity scales exponentially with synapse density. J. Neural Eng., in press) Brewer,GJ., Boehler, MD; Jones, TT, Wheeler, BC. 2008. NbActiv4 medium for neurons increases synaptic densities and network spike rates on multielectrode arrays compared to Neurobasal/B27. J. Neurosci. meth. 170:181-187 (PM 18308400)
Strategies for switching to NbActiv4® from Neurobasal/B27
NbActiv4 contains BrainBits (Transnetyx Tissue) Basal Medium and NS21. It can be stored at 4 °C for up to 6 months if unopened. It should not be frozen. Both media (Neurobasal/B27 and NbActiv4) are designed for use at 37 °C in a 5% CO2 incubator. Neurons are subject to less oxidative stress if the oxygen is also regulated at 9%. To evaluate NbActiv4 in the context of current experiments, we suggest a side-by-side comparison. For new lines of study, experiments can begin directly with NbActiv4. Beginning cultures in Neurobasal/B27 followed by a switch to NbActiv4 shows no indication of being better than use of NbActiv4 from the start. One-Half medium changes with incubator temperature equilibrated medium should be performed at least once a week, with more frequent changes at cell densities above 300 cells/mm2.
Brainbits货号NB4产品图片
