原理介绍
无铜点击化学反应，即菌株促进的叠氮炔环加成（SPAAC）是一种生物正交反应，利用一对试剂，即环辛基和叠氮化合物，它们彼此有效地反应，同时对羟基、氨基和硫醇等天然官能团保持惰性。SPAAC能够通过形成稳定的三唑，在水和其他复杂的化学环境中标记各种各样的生物分子，而无需任何辅助试剂。在众多已知的环辛基化合物中，二苯并环辛基化合物（DBCO）是一类具有相当快的动力学和良好的水缓冲稳定性的试剂。在生理温度和pH范围内，DBCO基团不会与许多生物分子中天然存在的巯基、氨基或羟基发生反应。DBCO-PEG4-琥珀酰亚胺酯是一种常用的通过氨基修饰将DBCO基团引入生物分子的试剂。靶点科技分销的ClickChemistryTools的DBCO-PEG4-NHS Ester，是高效率的DBCO 琥珀酰亚胺酯。
化学结构
参考文献
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