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Kerafast Anti-tdTomato [16D7] EST203

发布时间:2022-07-06   点击次数:78次

产品介绍:

Kerafast货号:EST203 Anti-tdTomato [16D7] Antibody该抗体针对重组蛋白而产生,可识别 tdTomato。


tdTomato 蛋白来源于 DsRed 的单体突变体,这是一种来自 Discosoma 属的所谓圆盘珊瑚的红色荧光蛋白。 tdTomato 是生物技术和细胞生物学中的重要工具,可作为光谱上不同的伴侣或绿色荧光蛋白(GFP;来自 Aequorea 水母)的替代品。


Kerafast EST203 Anti-tdTomato [16D7] Antibody:This antibody was raised against a recombinant protein and recognizes tdTomato.

The tdTomato protein was derived from a monomeric mutant of DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. tdTomato is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).


Product Type:Antibody
Antigen:Raised against a recombinant protein and recognizes tdTomato.
Isotype:IgG2a
Clonality:Monoclonal
Clone Name:16D7
Reactivity:tdTomato (also recognizes mCherry)
Species Immunized:Rat
Purification Method:Protein G
Buffer:0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications:WB (1:1000), ELISA, IF, IHC (1:100-500), IP (1ug antibody to 100ug total protein)
Storage:4C, -20C for long term storage
Shipped:Cold packs 


数据展示:


Immunohistochemistry using Anti-tdTomato [16D7] Antibody in a transgenetic mouse, weakly expressing Cre-driven tdTomato.

The Anti-tdTomato [16D7] Antibod with Alexa-555 secondary was used to visualize Cre+ cells for tdTomato expression. The protocol used is as follows: (1) Perfuse mouse with ~ 25 ml ice-cold normal ringer solution with pH 7.4. (2) Perfuse mouse with ~ 50 ml ice-cold fixative consisting of 2% PFA and 15% picric acid with pH = 7.4. (3) After brain extraction, post fix for one hour with 2% PFA + 15% picric acid. (4) Quickly wash brain 3x with liberal amount of PBS, thoroughly removing all fixative. (5) Slice in ice-cold ringer and section at 60 µm. Wash section 3x with 0.01M PBS, 15’ each time. (6) Block for 1 hours with 10% normal goat serum INGS), 3% Bovine serum albumin (BSA), and 0.1% Trixton-X in 0.01M PBS. (7) Incubate primary tdTomato Ab at 1:500 – 1:250 for 24 hours at 4°C on a shaker. (8) Extract primary solution without washing. (9) Incubate for 2 hours in room temperature secondary (Alexa-555) at 1:500 in the following solution made in 0.01M PBS: 1% NGS + 0.01% Triton-X. (10) Wash sections 3x, 15’ each time. (11) Mount section on microscope slide and cover slip using anti-fading preservative (e.g. Vectashield or Fluoromount-G). Image courtesy of Kerafast customer Vivian Hernandez from Dr. C. Savio Chan's Lab, Northwestern University, submitted February 23, 2015.



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靶点科技(北京)有限公司现货库存kerafast的货号EST203 Anti-tdTomato [16D7] Antibody。同时,这个抗体也是Anti-mCherry [16D7] Antibody,货号是EST202.

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