特色:
可用于检测 R 环
对 DNA-RNA 杂交体的高特异性和亲和力
不与单链 DNA 或双链 DNA 发生交叉反应
对于富含 AU 的双链 RNA,观察到了轻微的交叉反应(约 5 倍以下)。
长度为 8、10、15 和 23 个碱基对的杂交体显示出高亲和力结合
DNA-RNA 杂合体是真核细胞中的一种自然现象,这些杂合体的水平在具有高转录活性的位点增加,例如在转录起始、抑制和延伸期间。由于 RNA-DNA 杂合体会影响基因组的不稳定性,因此 S9.6 抗体是一种有用的试剂,可帮助研究在 DNA 复制或其他细胞过程中由这些杂合体形成的 R 环和损伤的后果。此外,S9.6 抗体可有效识别用于微阵列研究的 RNA-DNA 杂交。
This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
Highlights:
* Useful in the detection of R-loops
* High specificity and affinity for DNA-RNA hybrids
* Does NOT cross-react with single-stranded DNA or double-stranded DNA
* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.
* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length
产品详情:
Product Type: | Antibody |
Name: | Anti-DNA-RNA Hybrid [S9.6] |
Antigen: | S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen |
Isotype: | Rabbit IgG |
Fusion Tag(s): | Mouse Fab version contains His-tag |
Clone Name: | S9.6 |
Reactivity: | High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids |
Immunogen: | ΦX174 bacteriophage-derived synthetic DNA/RNA |
Purification Method: | Protein A/G |
Buffer: | ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300 |
Tested Applications: | Dot Blot Analysis: 0.2 µg/mL. |
参考文献:
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8. Graf M, Bonetti D, Lockhart A, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT, Luke B. Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle. Cell. 2017 Jun 29;170(1):72-85.e14.
9. Gorthi A, Romero JC, Loranc E, Cao L, Lawrence LA, Goodale E, Iniguez AB, Bernard X, Masamsetti VP, Roston S, Lawlor ER, Toretsky JA, Stegmaier K, Lessnick SL, Chen Y, Bishop AJR. EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. Nature. 2018 Mar 15;555(7696):387-391.
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