技术文章您现在的位置:首页 > 技术文章 > 如何使用SHI-FIX对悬浮细胞做免疫荧光?

如何使用SHI-FIX对悬浮细胞做免疫荧光?

发布时间:2022-10-15   点击次数:347次

您是否希望悬浮细胞的免疫荧光像贴壁细胞一样容易?现在是这样!Shi-fix™(由Shikhar生物技术公司制造)盖滑允许悬浮细胞分层或直接作为单层生长。简单,无忧的实验方案,无需离心柱即可将悬浮细胞固定到载玻片上。只需将细胞添加到盖玻片中,等待30分钟,用PBS洗涤未结合的细胞并继续免疫染色(或继续培养以获得所需的细胞密度)。

这些盖玻片也可用于染色悬浮细胞以进行显微镜检查,或用于固定悬浮细胞以进行共聚焦显微镜检查。更重要的是,如果需要,您还可以在细胞附着在盖玻片上时刺激它们。您可以像贴壁细胞一样处理非贴壁细胞!

Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).

These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!


SHI-FIX cover-slips玻片做的悬浮细胞免疫荧光实验图:


SHI-FIX cover-slips玻片的操作步骤:简单!方便!!


SHI-FIX cover-slips玻片的产品订购信息:





靶点科技(北京)有限公司

靶点科技(北京)有限公司

地址:中关村生命科学园北清创意园2-4楼2层

© 2022 版权所有:靶点科技(北京)有限公司  备案号:京ICP备18027329号-2  总访问量:117920  站点地图  技术支持:化工仪器网  管理登陆

QQ在线客服
联系方式

15210359867

4000043669