德国Emfret公司专注心血管和血液系统研究用抗体研发和生产,供应的抗体包括清除血液中血小板、流式细胞术鉴定分析小鼠血小板表面糖蛋白,emfret公司的抗体不仅适用于流失分析、WB检测,还可用于免疫组化/免疫荧光及免疫共沉淀检测。
背景资料:
This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice1,2 . Platelet depletion is frequently used to study the general role of platelets in (patho-) physiology3 .
保存条件:
The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography.
Stable for six months from date of shipment when stored at 4°C.
KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.
应用:
This preparation is optimized to reduce the platelet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).
同型对照(抗体来源): Rat IgG (Wistar)
缓冲液: 0.5 mg purified immunoglobulins in PBS, 0.2% (w/v) BSA, sterile filtered
浓度: 0.5 mg/ml
产品图片:
实验结果:
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