Azido-PEG4-NHS Ester叠氮基-PEG4-NHS 酯是一种标记试剂,可用于通过稳定的酰胺键修饰具有叠氮基团的伯胺基团(例如赖氨酸残基的侧链或氨基硅烷包被的表面)。叠氮化物 (N3) 基团通过 CuAAC 与末端炔烃反应反应,通过无铜点击反应与应变环辛炔(例如 DBCO 或 BCN 化合物)反应,或通过称为 Staudinger 化学的机制与磷化氢标记分子反应,从而实现生物样品中衍生化分子的高效和特异性偶联。
Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.