生物素-PEG3-叠氮化物是一种不可裂解的叠氮化物活化生物素化试剂，通过铜催化的点击反应与末端炔烃反应反应，通过无铜点击反应与应变的环辛炔（例如DBCO或BCN化合物）反应，或通过称为Staudinger化学的机制与磷化氢标记的分子反应，从而实现生物样品中衍生化分子的高效和特异性偶联。这些反应是生物正交的，或者换句话说，与生物系统相容，因为它的成分不与生物环境发生反应。
Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. These reactions are bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.
