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Broadpharm生物素化试剂选择指南

更新时间:2023-12-21   点击次数:387次

生物素-亲和素相互作用通常用于检测和/或纯化蛋白质,因为这两种分子彼此具有高度特异性。生物素化是将生物素附着在蛋白质和其他大分子上的过程。

在选择生物素化试剂时,用户需要考虑以下几个方面。

The biotin–avidin interaction is commonly exploited to detect and/or purify proteins because of the high specificity that these two molecules have for each other. Biotinylation is the process of attaching biotin to proteins and other macromolecules.

When selecting the biotinylating reagents, the user need consider the following aspects.


    BroadPharm has all kinds of biotinlyation reagents for meet the need of your advanced research. The variability of these reagents substantially expands the range of applications for avidin–biotin chemistry

  • *Varieties of biotin functional group reagents differ in length, solubility, cell permeability and cleavability.

  • *Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.

  • *Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF.

  • *Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable.

BroadPharm拥有各种生物素化试剂,以满足您高级研究的需求。这些试剂的可变性大大扩展了亲和素-生物素化学的应用范围

*各种生物素官能团试剂的长度、溶解度、细胞通透性和裂解性各不相同。

*含有二硫键的品种可以使用还原剂进行裂解,使生物素基团能够与标记的蛋白质断开。

*非磺化NHS-生物素具有细胞渗透性,但必须溶解在有机溶剂中,如DMSO或DMF。

*Sulfo-NHS生物素(以及那些带有聚乙二醇化间隔物的生物素)是直接水溶性的,但不能渗透膜。





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