B-lymphoma cells悬浮细胞，B细胞淋巴瘤是B细胞发生的实体肿瘤。包括霍奇金淋巴瘤和非霍奇金淋巴瘤。B细胞淋巴肿瘤是一种起源于B淋巴细胞的恶性肿瘤，也被称为非霍奇金淋巴瘤（NHL）。它是淋巴瘤的一种常见类型，约占所有淋巴瘤的30-40%。B细胞淋巴肿瘤的特点是异常B细胞在淋巴结或其他器官中异常增生，并可能影响患者的免疫功能。
研究悬浮细胞时，我们经常需要做免疫荧光，但是悬浮细胞如何固定？一直困扰着我们。如何像贴壁细胞一样处理悬浮细胞，避免掉片或者细胞固定不住，痛点解决方案来了。靶点科技Shi-Fix coverslips可以固定悬浮细胞，仅仅需要四步。
B-lymphoma cells如何固定？并且固定还可以刺激培养，可以参考如下解决方案。
B-lymphoma cells (5 × 105 cells/mL) were seeded in Shi-fix coverslips (Shikhar Biotech) and incubated in RPMI 1640 medium supplied with 10% FBS under a 5% CO2 atmosphere. After 24 h of incubation, the cells were washed twice with PBS and treated with FITC-labeled NK20a peptide (NK20a-FITC) or NK20a-FITC@Au nanoparticles for 1 h at 37 °C. For visualization, the cell membranes were stained with Cytopainter (ab219942, Abcam, Cambridge, UK), and the nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI). The samples were each treated with 100 µL of Cytopainter for 20 min, then washed with fresh PBS. Subsequently, the cells were fixed with 4% glutaraldehyde for another 20 min. After the fixation, 3–4 drops of mounting medium with 0.0002% DAPI (ab104139, Abcam, Cambridge, UK) were applied directly to the samples to stain the nuclei of cells. The prepared samples were imaged with a confocal fluorescence microscope (C2si + , Nikon, Tokyo, Japan).
