大肠杆菌自诱导培养基适用于由功能性的lac操纵子(包括lac Y和lac Z)控制的表达系统，如PET系列。表达菌种可用BL21(DE3)。大肠杆菌自诱导培养基(E.coli Auto-Induction Medium)是在LB培养基的基础上进行改进优化配方，可用于lac调控表达的大肠杆菌菌株培养及蛋白表达。含有多种营养物质并额外添加微量元素、氨基酸等，碳源梯度设计既能满足菌体生长需求，同时能实现外源蛋白在细菌达到饱和生长期后的自动诱发。同等产量相比传统LB使用更少培养体积，无需密切监控菌液密度的过程(测OD)，且无需额外添加IPTG。本品同样适用于多克隆表达，高通量选。产品为粉末形式，500g/瓶，按实验室已经习惯的常规LB培养基样配制，配制方便。
LB自诱导培养基LB Broth (Auto Induction Medium)产品介绍选购指南
用于制备不含微量元素的LB培养基的固体粉末，添加有葡萄糖和α-乳糖，以便在大肠杆菌中通过IPTG诱导的启动子自动诱导蛋白表达。
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.
Preparation:
Add 34,85g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.
Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
MgSO4: 0,15
(NH4) 2SO4: 3,30
KH2PO4: 6,80
Na2HPO4: 7,10
Glucose 0,50
Alpha Lactose 2,00
Final pH (25ºC): 7,0 ± 0,2
