肿瘤相关巨噬细胞（TAMs）是存在于肿瘤微环境中的一类免疫细胞，具有双重作用：既可抑制肿瘤生长，也可促进肿瘤进展。它们主要来源于外周血单核细胞，受肿瘤分泌的趋化因子招募，并通过不同表型（如促炎的M1型和抑炎的M2型）影响肿瘤发展。目前，靶向TAMs已成为癌症治疗的研究热点。促炎的M1型和抑炎的M2型巨噬细胞极化的两种典型状态的检测，可以订购靶点科技Cell-Out® M1巨噬细胞标记和示踪荧光探针，Cell-Out® M2巨噬细胞标记和示踪荧光探针。
使用荷兰Liposoma巨噬细胞清除剂clodronateliposomes氯膦酸盐脂质体来清除肿瘤巨噬细胞，是在动物模型中研究巨噬细胞功能的重要手段之一。对于细胞接种动物肿瘤模型，一般持续要1周到4周不等。根据肿瘤细胞类型，小鼠背景，小鼠基因遗传操作，肿瘤细胞修饰和改造，接种方式，关注阶段等具体实验的设计。巨噬细胞清除剂的给药方案都不尽相同。如下这篇Nature Communications文献，可以参考。
The evaluation of clodronate treatment on tumor progression of CI-competent and deficient tumors. (a) Tumor growth curves [n=6, df=10, t(day 35)=5.59] of HCT+/+ xenografts in ICRF nude mice treated with or without clodronate. Representative tumors are shown. Scale bar: 1 cm. (b) Haematoxylin/Eosin (HE) staining of HCT+/+ xenografts in ICRF nude mice treated with or without clodronate. Scale bars: 50 µm. Data are mean±s.e.m. (c) Tumor growth curves [data were log transformed, n=6, df=10, t(day33)=3.3] and excised tumor volume (n=6, df=10, t=2.5) of 143B-/- xenografts in Rag1-/-FVB mice treated with or without clodronate. Data are mean±s.e.m. Representative tumors are shown. Scale bar: 2 cm. (d) Representative images of immunofluorescent staining analyzing vessel morphology in 143B-/- xenografts treated with or without clodronate (n=4, df=6, t=3.16).
论文信息：
论文题目：Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses
期刊名称：Nature Communications
时间期卷：10, Article number: 903(2019)
在线时间：2020年2月22日
DOI： doi.org/10.1038/s41467-019-08839-1
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes& Control Liposomes
办事处：Target Technology(靶点科技)
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除肿瘤相关巨噬细胞的材料和方法：
For the clodronate treatment experiments in Fig. 7c and Supplementary Fig. 17a–d, the animals were pre-injected intraperitoneally with PBS or clodronate liposomes (100 µL, ClodronateLiposomes, Liposoma BV) on the day prior to cell injection. On the day of tumor cell injection, 5 × 106 cells in growth factor reduced matrigel (100 µL) were injected subcutaneously, immediately followed by injection of 40 µL of PBS or clodronate liposomes at the same position. The mice continued to receive intraperitoneal injection of liposomes twice weekly (100 µL).
材料和方法文献截图：
