中文摘要：
基于益生菌的结直肠癌（CRC）生物疗法因肿瘤靶向能力差和复杂肿瘤微环境中生物活性有限而面临重大挑战。本研究显示，通过锰能够增强乳酸乳球菌（Lactobacillus reuteri，L. reuteri）固有的生物活性，包括增殖、代谢和结肠定植，从而促成了锰工程菌株（MnLR）的构建。口服给药MnLR及其代谢底物甘油（MnLR/Gly）可促进L. reuteri及其抗肿瘤代谢产物在结肠肿瘤中的富集。在多种前临床结肠肿瘤模型中，单独使用MnLR/Gly即可实现95.6%的原位肿瘤生长抑制、肝转移减少62.1%，并提供持久保护，75%的预防性处理小鼠长期无肿瘤，62.5%对肿瘤再挑战具有抵抗力。从机制上看，MnLR/Gly可诱导肿瘤内及外周树突状细胞成熟、M1型巨噬细胞极化以及效应T细胞反应。在原位CRC兔模型中，GlyMnLR肠溶胶囊显示出与标准化疗相当的显著抗肿瘤效果，同时表现出良好的安全性。这些发现表明，MnLR是一种有效的单独益生菌疗法，用于CRC的预防和治疗。
英文摘要：
Probiotic-based biotherapy for colorectal cancer (CRC) faces significant challenges due to poor tumor-targeting and limited bioactivities within the complex tumor microenvironment. This study shows that Lactobacillus reuteri (L. reuteri)’s inherent bioactivities, including proliferation, metabolism, and colorectal colonization, can be enhanced by manganese, prompting the fabrication of a manganese-engineered strain (MnLR). Oral administration of MnLR with its metabolic substrate-glycerol (MnLR/Gly) promotes the enrichment of L. reuteri and its antitumor metabolites within colon tumors. Across multiple preclinical colon tumor models, MnLR/Gly alone achieves a 95.6% inhibition of orthotopic tumor growth, liver metastases reduction by 62.1%, and confers durable protection, with 75% of prophylactically treated mice remaining long-term tumor-free and 62.5% resisting tumor rechallenge. Mechanistically, MnLR/Gly induces both intratumoral and peripheral dendritic cell maturation, M1 macrophage polarization, and effector T-cell responses. In orthotopic CRC rabbit models, GlyMnLR enteric capsules demonstrate significant antitumor efficacy comparable to standard chemotherapy while exhibiting favorable safety. These findings highlight MnLR as an effective standalone probiotic therapy for CRC prevention and treatment.
论文信息：
论文题目：Manganese-engineered Lactobacillus Reuteri with enhanced antitumor and immunomodulatory activities for colorectal cancer prevention and treatment
期刊名称：Nature Communications
时间期卷：17, Article number: 761(2026)
在线时间：2025年12月19日
DOI： doi.org/10.1038/s41467-025-67437-6
产品信息：
货号：C-005
规格：5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes氯膦酸盐脂质体
办事处：靶点科技
Clodronate Liposomes氯膦酸盐脂质体在小鼠结肠癌模型清除巨噬细胞和外周单核巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于Nature Communications：锰工程乳杆菌增强抗肿瘤和免疫调节活性用于结直肠癌的预防和治疗。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除肺泡巨噬细胞和单核巨噬细胞的材料和方法：
clodronate-encapsulated liposomes (200 μl per mouse; LIPOSOMA, Netherlands) on days 6, 2, and 1 before tumor inoculation, and on days 4, 8, and 12 following tumor inoculation.
材料和方法文献截图：
