中文摘要：
在医院环境中，脱水与营养不良问题十分常见，且往往存在诊断不足的情况。多重耐药菌感染每年造成超过 3.5 万名院内患者死亡。饮食与限水干预（DWR）对机体易感多重耐药病原体的影响尚不明确。本研究发现，饮食与限水会显著增加机体对 ESKAPE 耐药致病菌全身感染的易感性。
本研究采用耐甲氧西林金黄色葡萄球菌感染的小鼠血流感染模型，证实饮食与限水会显著提升小鼠的死亡率与发病程度。该干预会导致细菌载量升高、组织严重病变，同时肾脏内吞噬细胞数量增多。饮食与限水可改变吞噬细胞的功能，使其无法有效控制感染。
机制层面研究表明，饮食与限水会损害巨噬细胞对多种致病菌的吞噬能力，以及对凋亡中性粒细胞的胞葬作用。综上，本研究凸显了饮食与机体水合状态在抵御感染过程中发挥的关键作用。
英文摘要：
Dehydration and malnutrition are common and often underdiagnosed in hospitalsettings. Multidrug-resistant bacterial infections result in more than 35,000 deatha year in nosocomial patients. The effect of temporal dietary and water restriction(DWR) on susceptibility to multidrug-resistant pathogens is unknown. We reportthat DWR markedly increased susceptibility to systemic infection by ESKAPEpathogens. Using a murine bloodstream model of methicillin-resistantStaphylococcus aureus infection, we show that DWR leads to significantly increasemortality and morbidity. DWR causes increased bacterial burden, severe patholog:and increased numbers of phagocytes in the kidney. DWR appears to alter thefunctionality of these phagocytes and is therefore unable to control infection.Mechanistically, we show that DWR impairs the ability of macrophages to phago-cytose multiple bacterial pathogens and efferocytose apoptotic neutrophils.Together, this work highlights the crucial impact that diet and hydration play inprotecting against infection.
论文信息：
论文题目：Dietary and water restriction leads to increased susceptibility to antimicrobial resistant pathogens
期刊名称：Science Advances
时间期卷：Vol 10, Issue 30(2024)
在线时间：2024年7月24日
DOI: 10.1126/sciadv.adi7438
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：靶点科技
Clodronate Liposomes氯膦酸盐脂质体在细菌感染妊娠小鼠模型种清除巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于Science Advances：饮食与饮水限制会增加机体对抗菌药物耐药病原体的易感性。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除巨噬细胞的材料和方法：
Macrophage depletion was achieved by intravenous injection of clodronate-containing liposomes (1 mg of clodronate per mouse) or PBS liposomes (Liposoma) 24 hours before challenge with TH16.
巨噬细胞清除材料和方法文献截图：
