中文摘要：
目前学界对大脑受损血管修复过程中的细胞调控机制尚不明确，针对糖尿病等存在血管病变高危因素人群的相关研究尤为匮乏。本研究剖析了脑组织固有小胶质细胞与浸润性巨噬细胞在破损脑微血管修复进程中的作用。
研究结合活体延时成像、基因表达分析及免疫组化技术，鉴定出一类独特的半乳糖凝集素 3（Gal3）阳性吞噬型巨噬细胞；该细胞群与固有小胶质细胞存在明显区别，会浸润并聚集于糖尿病小鼠的血管损伤部位，且与微血管清除过程密切相关。在糖尿病小鼠体内使用荷兰Liposoma氯膦酸盐脂质体清除这类浸润性巨噬细胞后，细胞吞噬活性显著下降，同时能够抑制损伤后微血管的丢失。
上述研究结果揭示了浸润性 Gal3 阳性巨噬细胞在脑损伤后介导血管清除的全新作用，也为后续开展相关研究提供依据：明确清除该类细胞是否能够促进高危人群的脑血管修复。
英文摘要：
The cellular events that dictate the repair of damaged vessels in the brain, especially in those with vascular risk factors such as diabetes, is poorly understood. Here, we dissected the role of resident microglia and infiltrative macrophages in determining the repair of ruptured cerebral microvessels. Using in vivo time-lapse imaging, gene expression analysis, and immunohistochemistry, we identified a unique population of phagocytic Galectin 3 (Gal3) expressing macrophages, distinct from resident microglia, which infiltrated and aggregated at the site of injury in diabetic mice and were associated with the elimination of microvessels. Depletion of these infiltrative macrophages in diabetic mice attenuated phagocytic activity and prevented the loss of blood vessels after injury. These findings highlight a previously unknown role for infiltrative Gal3 expressing macrophages in promoting vessel elimination after brain injury and provide impetus for future studies to determine whether depleting these cells can facilitate vascular repair in at risk populations.
论文信息：
论文题目：Invasion of phagocytic Galectin 3 expressing macrophages in the diabetic brain disrupts vascular repair
期刊名称：Science Advances
时间期卷：Vol 7, Issue34(2021)
在线时间：2021年8月18日
DOI: 10.1126/sciadv.abg2712
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：靶点科技
Clodronate Liposomes氯膦酸盐脂质体静脉注射，清除外周巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于Science Advances：吞噬型半乳糖凝集素 3 阳性巨噬细胞浸润糖尿病小鼠脑组织会阻碍血管修复。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除巨噬细胞的材料和方法：
In vivo macrophage depletion
For drug intervention experiments, mice received intravenous CLR (50 mg/kg; Liposoma B.V.) 2 days before CMB induction, on the day of CMB induction, and 2 days later. This treatment regimen ensured sustained depletion of peripheral macrophages. Diff-Quik histological staining confirmed depletion of circulating immune cells in treated animals.
药物干预实验中，于脑微出血造模前 2 天、造模当日及造模后 2 天，对小鼠尾静脉注射氯膦酸二钠脂质体（CLR，给药剂量 50 mg/kg，厂商：Liposoma B.V.）。该给药方案可实现外周巨噬细胞的持续性清除。经迪夫快速（Diff-Quik）组织染色验证，给药小鼠外周循环免疫细胞已被有效清除。
巨噬细胞清除材料和方法文献截图：吞噬型半乳糖凝集素 3 阳性巨噬细胞浸润糖尿病小鼠脑组织会阻碍血管修复
