中文摘要：
GPR37在二十多年前被发现，但其生物学功能仍然知之甚少。在这里，我们报道了GPR37在多种感染和败血症模型中的保护作用。缺失Gpr37的小鼠在受到脂多糖（LPS）、李斯特菌以及鼠疟原虫伯格氏疟原虫（Plasmodium berghei）攻击后表现出死亡率增加和/或体温下降。野生型小鼠在LPS和李斯特菌诱导的败血症中，使用青蒿琥酯（ARU）和神经保护素D1（NPD1）可以获得保护，但这些药物的保护作用在Gpr37−/−小鼠中丧失。值得注意的是，我们发现ARU可以结合巨噬细胞中的GPR37，并促进病原体的吞噬和清除。此外，巨噬细胞通过荷兰Liposoma的巨噬细胞清除剂来清除会加重感染、败血症及其后续影响，而采用NPD1或ARU诱导的巨噬细胞移植可以降低感染、败血症和类疼痛行为。我们的研究揭示了ARU通过激活巨噬细胞在宿主细胞中的生理作用，并提示GPR37激动剂可能有助于治疗败血症、细菌感染和疟疾。
英文摘要：
GPR37 was discovered more than two decades ago, but its biological functions remain poorly understood. Here we report a protective role of GPR37 in multiple models of infection and sepsis. Mice lacking Gpr37 exhibited increased death and/or hypothermia following challenge by lipopolysaccharide (LPS), Listeria bacteria, and the mouse malaria parasite Plasmodium berghei. Sepsis induced by LPS and Listeria in wild-type mice is protected by artesunate (ARU) and neuroprotectin D1 (NPD1), but the protective actions of these agents are lost in Gpr37−/− mice. Notably, we found that ARU binds to GPR37 in macrophages and promotes phagocytosis and clearance of pathogens. Moreover, ablation of macrophages potentiated infection, sepsis, and their sequelae, whereas adoptive transfer of NPD1- or ARU-primed macrophages reduced infection, sepsis, and pain-like behaviors. Our findings reveal physiological actions of ARU in host cells by activating macrophages and suggest that GPR37 agonists may help to treat sepsis, bacterial infections, and malaria.
论文信息：
论文题目：Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice
期刊名称：Nature Communications
时间期卷：12, Article number: 1704 (2021)
在线时间：2021年3月17日
DOI：doi.org/10.1038/s41467-021-21940-8
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes
办事处：Target Technology(靶点科技)
Clodronate Liposomes氯膦酸盐脂质体清除腹腔巨噬细胞，疾病模型为：脂多糖（LPS）、李斯特菌以及鼠疟原虫伯格氏疟原虫（Plasmodium berghei）攻击。荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes见刊于Nature Communications：在巨噬细胞中激活GPR37可对感染引起的小鼠败血症和类似疼痛行为提供保护.
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法：
We obtained the following reagents from the indicated sources/vendors: pHrodo® Red Zymosan Bioparticles® Conjugate (Thermo Scientific; P35364, 1 mg/ml PBS stock), pHrodo® Red AM (Thermo Scientific; P35372, 10 mM DMSO stock), natural drug library (Selleckchem; L1400, 10 mM DMSO stock, see Supplementary Table 1 for drugs tested), LC3 detection dye (Dojindo Molecular Technologies, Inc.; D675-10), ROS/RNS kit (ENZO Life Science; ENZ-51001-200), artesunate (Cayman Chemicals; 11817, 30 mM DMSO stock), artemisinin (Cayman Chemicals; 11816, 30 mM DMSO stock), dihydroartemisinin (Abcam; ab142690, 30 mM DMSO stock), and clodronate liposomes (Liposoma B.V.; C-005). NPD1 was gifted from Resolvyx Pharmaceuticals. All reagents were used in the dose and manner described in the figure legends.
材料和方法文献截图：
