中文摘要：
在睾丸中，间质巨噬细胞被认为在胎儿发育期间来源于卵黄囊，随后被骨髓来源的巨噬细胞取代。相比之下，据报道围小管巨噬细胞首先在出生后睾丸中出现，并且代表骨髓来源单核细胞的后代。在这里，我们使用高维单细胞分析定义了胎儿和出生后睾丸中新型的单核细胞和巨噬细胞类型。我们的结果显示，间质巨噬细胞主要来源于胎肝衍生的前体细胞，而围小管巨噬细胞则在出生时由胚胎前体细胞生成。我们发现，即使在系统性巨噬细胞耗竭后，骨髓来源的单核细胞也不会对睾丸巨噬细胞库的补充做出显著贡献。胎儿期而非出生后存在的巨噬细胞对于正常的精子生成是必要的。因此，我们的多方面数据通过阐明睾丸巨噬细胞的分化、稳态和功能，挑战了当前睾丸巨噬细胞生物学的现有范式。
英文摘要：
In the testis, interstitial macrophages are thought to be derived from the yolk sac during fetal development, and later replaced by bone marrow-derived macrophages. By contrast, the peritubular macrophages have been reported to emerge first in the postnatal testis and solely represent descendants of bone marrow-derived monocytes. Here, we define new monocyte and macrophage types in the fetal and postnatal testis using high-dimensional single-cell analyses. Our results show that interstitial macrophages have a dominant contribution from fetal liver-derived precursors, while peritubular macrophages are generated already at birth from embryonic precursors. We find that bone marrow-derived monocytes do not substantially contribute to the replenishment of the testicular macrophage pool even after systemic macrophage depletion. The presence of macrophages prenatally, but not postnatally, is necessary for normal spermatogenesis. Our multifaceted data thus challenge the current paradigms in testicular macrophage biology by delineating their differentiation, homeostasis and functions.
论文信息：
论文题目：Generation, localization and functions of macrophages during the development of testis
期刊名称：Nature Communications
时间期卷：11, Article number: 4375 (2020)
在线时间：2020年9月1日
DOI： doi.org/10.1038/s41467-020-18206-0
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：Target Technology(靶点科技)
Clodronate Liposomes氯膦酸盐脂质体联合抗体清除骨髓，骨髓来源的血液单核，还有重点研究的部位睾丸巨噬细胞，荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes见刊于Nature Communications：睾丸发育过程中巨噬细胞的产生、定位及功能。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除睾丸巨噬细胞的材料和方法：
Ten-day-old wild-type mice were treated with CSF1 neutralizing antibody and clodronate-containing liposomes to deplete tissue macrophages and blood monocytes. Intraperitoneal injection of CSF1 antibody (Bio X Cell, clone 5A1) or IgG1 isotype control (Bio X Cell, clone HRPN) (500 µg on the first cycle, 250 µg on the second and third cycles) was followed by intravenous administration of clodronate-containing or empty control liposomes (Liposoma; in 50 µl volume) on a subsequent day. Three consecutive treatment cycles were performed at three-day intervals. Tissues were harvested for flow cytometry and imaging analyses 40 h, 11 days, or 21 days after the last clodronate injection
材料和方法文献截图：
