中文摘要：
开发有效的金黄色葡萄球菌（S. aureus）疫苗一直是一项具有挑战性的工作，过去多年众多失败的临床试验也证明了这一点。在本研究中，我们配制了一种包含高度保守的“兼职"蛋白——丙酮酸脱氢酶复合物E2亚基（PDHC）的疫苗，并证明该疫苗在多种小鼠疾病模型中能够诱导针对流行病学相关葡萄球菌菌株的保护性免疫。虽然抗体应答有助于控制细菌，但在血流感染模型中，抗体对于保护性免疫并非必需。相反，疫苗诱导的全身性免疫依赖于γδ T细胞。此前有研究认为，先前接触过金黄色葡萄球菌可能会降低疫苗效力。然而，PDHC诱导的保护性免疫在先前接触过金黄色葡萄球菌的小鼠中仍能促进细菌清除。综上所述，我们的研究结果表明，PDHC是一种有前景的、不依赖血清型的候选疫苗，对甲氧西林敏感和耐甲氧西林的金黄色葡萄球菌分离株均有效。
英文摘要：
Developing an effective Staphylococcus aureus (S. aureus) vaccine has been a challenging endeavor, as demonstrated by numerous failed clinical trials over the years. In this study, we formulated a vaccine containing a highly conserved moonlighting protein, the pyruvate dehydrogenase complex E2 subunit (PDHC), and showed that it induced strong protective immunity against epidemiologically relevant staphylococcal strains in various murine disease models. While antibody responses contributed to bacterial control, they were not essential for protective immunity in the bloodstream infection model. Conversely, vaccine-induced systemic immunity relied on γδ T cells. It has been suggested that prior S. aureus exposure may contribute to the reduction of vaccine efficacy. However, PDHC-induced protective immunity still facilitated bacterial clearance in mice previously exposed to S. aureus. Collectively, our findings indicate that PDHC is a promising serotype-independent vaccine candidate effective against both methicillin-sensitive and methicillin-resistant S. aureus isolates.
论文信息：
论文题目：Conserved moonlighting protein pyruvate dehydrogenase induces robust protection against Staphylococcus aureus infection
期刊名称：PNAS
时间期卷：21 (36) e2321939121
在线时间：2020年5月22日
DOI: 10.1073/pnas.2321939121
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes&Control Liposomes
办事处：靶点科技
Clodronate Liposomes氯膦酸盐脂质体清除小鼠金黄色葡萄球菌感染模型里巨噬细胞。荷兰Liposoma巨噬细胞清除剂ClodronateLiposomes见刊于PNAS：保守型兼职蛋白丙酮酸脱氢酶诱导针对金黄色葡萄球菌感染的强效保护。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体清除巨噬细胞的材料和方法：
In vivo macrophage depletion
For macrophage depletion, mice were injected intravenously with 100 µL of clodronate liposome (Liposoma) suspension/10 grams of mouse weight 2 d before S. aureus challenge. The same amount of control liposome was administered to the control group.
巨噬细胞清除材料和方法文献截图：
